未分类 – 杭州百伴生物技术有限公司

Solve the frozen storage tube 2D code frost problem: principle analysis and Bann solution

BANN — Fri, 11 Oct 2024 02:11:17 +0000

In recent years, in order to better meet the needs of scientific research, biopharmaceuticals, biological samples library and other high-throughput storage, more and more organizations have constructed an ultra-low-temperature environment system for sample storage mainly based on -80℃ ultra-low-temperature refrigerator. When the samples are put into the refrigerator and then taken out, frost will appear at the bottom of the freezing tube, resulting in a lower success rate of reading the QR code at the bottom of the tube, which affects the work efficiency. Freezing tube is a plastic product, the surface is usually smooth or has a slight bump, these characteristics are easy to capture the tiny water droplets in the air. When the temperature drops, these water droplets will quickly condense into ice crystals and form frost. In order to solve the problem of frost at the bottom of the cryopreservation tubes from the root, BioBest is guided by the integrated in-mold labeling process developed by BioBest, which improves the bottom structure and provides more effective solutions for customers.

In the process of developing the solution, Biobay’s engineers started from the principle of frosting bottom layer, and studied how to inhibit the formation of condensation nucleus at the bottom of the tube. Condensation nucleus (condensation nucleus) refers to the solid, liquid and gaseous aerosol particles that play the role of condensation core in the condensation process. Condensation nucleus in the process of frost on the surface of a rough object, the roughness of the surface has a significant effect on the formation and distribution of frost. Rough surfaces usually have more tiny depressions and protrusions, and these tiny topographical features provide more attachment points for condensation nuclei. When water vapor in the air comes into contact with these rough surfaces, condensation nuclei are easily trapped and lodge in these irregular surface structures.

Rough surfaces are more likely to attract condensation nuclei than smooth surfaces. This is due to the fact that the unevenness of the surface increases the contact area, making it easier for tiny airborne particles to be deposited on it. This adsorption not only increases the number of condensation nuclei on the surface, but also makes the condensation process of water vapor on these nuclei more efficient, which in turn promotes the rapid formation and accumulation of frost. In addition, surface roughness has an important influence on the initial stage of frost crystal formation. Tiny grooves in rough surfaces are more likely to collect initial water droplets when the temperature drops just below freezing. These small droplets preferentially form frost crystals within the grooves as they gradually cool and freeze. This process reflects the “guiding effect” of the rough surface on the formation of frost crystals, i.e. its structural characteristics determine the initial location and shape of the frost crystals.

Bann cryopreservation tube adopts self-developed integrated intra-film labeling process to pre-print the bottom two-dimensional code on the polymer material, and then the printed image label is directly placed into the predefined molding area on the mold, so that the label and the container are fused together in the process of product injection molding, and the label is flush with the surface of the product. This process compared to the mainstream laser etching code-making process can make the bottom of the tube to achieve better integrity, thus fundamentally solve the problem of rough bottom of the tube due to etching so that the bottom of the tube adsorption of more condensation nuclei, thus causing more serious frost problems.

In addition, Bann cryopreservation tubes provide a solution to the frost problem while maintaining the safety and quality of the SBS automation standard with high precision by covering the bottom surface with a protective layer made of polymer material. This protective layer is sufficient to meet the needs of wear-resistant under normal use, and can show good resistance under extreme working conditions such as organic solvent etching, high temperature steam sterilization, liquid nitrogen cryogenic freezing and ultrasonic cleaning.

The integrated in-film labeling process developed by Bann can provide a highly efficient solution to the inefficient decoding problems caused by frost under a system that meets high-precision automation standards.

Hangzhou Biobank Bio-tech | Receives the First Domestic Sales Order of Full Rack Cryopreservation Tube Capper/Decapper in China

BANN — Tue, 16 May 2023 02:58:53 +0000

On July 28, the official website of the Fifth Affiliated Hospital of Sun Yat-sen University released two procurement project result announcements: Hangzhou Biobank Bio-technology Co., Ltd. won the bidding of 550,000 SBS standard four-code all-in-one cryopreservation tubes procurement project and the first domestic procurement project of domestic whole-plate cryopreservation tubes uncapping device.

Prefabricated Encoders

Cryopreservation tubes are mainly used for transportation and preservation of biological samples in ultra-low temperature refrigerators (-80°C) and liquid nitrogen tanks (-196°C). Most of the general-purpose cryopreservation tubes are arranged in 9×9 or 10×10 format, with a capacity of 2mL or 1.5mL, which cannot be adapted to the batch processing of automated equipment (pipetting workstations, automatic cap closure, automated refrigerators, etc.), and can only be used for the manual pre-processing of samples, which is suitable for the management of small amount of sample storage.

For high-throughput pre-processing of biological samples, only SBS (Society for Biomolecular Sciences) standardized cryopreservation tubes can be used as carriers. This standard, published by the American National Standards Institute (ANSI) on behalf of SBS in 2004, governs various characteristics of microtiter plates (and similar biosample carriers), including well dimensions (e.g., diameter, spacing, and depth) as well as microtiter plate characteristics (e.g., size and stiffness) (standardized dimensions of 127.76 mm x 85.48 mm), allowing interoperability between microtiter plates (and similar biosample carriers), instrumentation, and the use of the SBS standard. carriers), interoperability between instruments and equipment from different vendors, globally available laboratory automated processing equipment is designed and manufactured to ANSI/SBS standards.

At present, there are hundreds of domestic and foreign manufacturers producing SBS standard cryotubes, but there are no more than ten manufacturers worldwide that can produce automatic whole plate opener. Many manufacturers understand that the purpose of users choosing SBS standard cryopreservation tubes is for high-throughput sample processing, and they are also actively developing matching automatic whole plate opener. However, the difficulty of development is not only in the structure and debugging of the equipment, the ability to carry out the whole plate switch cap of the cryopreservation tube in the product design, production process, quality control and other aspects of the high requirements, only to ensure a high degree of consistency of the product, in order to complete the 96pcs / 48pcs of cryopreservation tubes dozens of times the switch is as smooth as new, the cap will not fall, and there will be no high and low unevenness. Therefore, only when the product precision and uniformity of the cryopreservation tubes meet the strict requirements, can they be actually adapted to the automatic cap opener, rather than just “looking like” SBS cryopreservation tubes can be used.

The research and development of BANN BM series of automatic whole plate opener took 3 years, during this period, not only the mechanical and electrical structure of the automatic opener is designed and improved, but also in the process of research and development to push back the design of cryopreservation tubes, technology, quality control enhancement and improvement. The mechanical design of the cap snap point of the cryopreservation tube, the production process to keep the tolerance of the inner diameter within ±0.02mm, the algorithm setting of the opening and closing action of the cap, and the tens of thousands of tests in the automated assembly workshop …… a series of standards to meet the final guarantee of the accuracy and stability of the equipment.

Considering the limitations of laboratory space and the actual needs of experimental operations, Byam Bio also continuously optimizes the cap opener, and under the premise of completing the same function, the volume and weight are far better than similar products in the market, and can be put into the biological safety cabinet for operation, without the need to design and customize non-standard equipment. 2023 April, BANN BM series of automatic cap openers and closers have obtained the CE certification, and have been successfully sold to Europe and the United States; in July, finally won the first domestic order, the bidding party is the famous three hospitals in China, the Fifth Hospital. In April 2023, BM series automatic cap opener obtained CE certification and was successfully sold to Europe and the United States; in July, it finally got the first domestic order, and the bidder was the famous three hospitals in China, the Fifth Hospital of CUHK, and BM series of products has become the only domestic whole-plate cap opener for cryopreservation tubes that has realized commercial sales in the market up to now.

The CU5 Biological Sample Bank is the Zhongshan University Precision Medicine Science Center Biological Sample Bank Zhuhai Demonstration Bank was built in 2017, and the type of preservation covers non-infectious clinical samples and marine medicine research samples, and in the future, it will strive to realize the unified processing, centralized management, individualized storage, and resource sharing of biological samples of the Guangdong, Hong Kong and Macao Bay Area region, and provide medical sample resources for the extensive development of large populations of multi-histology studies, prospective cohort studies, marine medicine special population diseases, etc. to provide medical sample resources. After being approved for the first time in 2018 for the “Approval of China’s Human Genetic Resources Preservation” administrative licensing program, CUHK-5 sample bank will again be approved for this national administrative licensing qualification in 2022. After the approval, the bank will have 11 sample types (urine, feces, saliva, etc.) and sample-related data information (including clinical information, high-throughput sequencing information, and various types of histological information, etc.), instead of only two types of samples, namely, whole blood and tissues.

After China passed the Biosafety Law of the People’s Republic of China in 2020, the construction of biospecimen libraries (viral libraries, human remains resources, chronic disease cohorts, etc.) began to be emphasized in various parts of the world. However, at present, the construction of biospecimen libraries focuses on the investment of hardware equipment, and according to the past experience, it is thought that there are a lot of manufacturers of freeze-storage tubes for preserving samples, which is a kind of consumable product with strong generalizability and substitutability, and the demand for high-throughput processing after the construction of the libraries is not often taken into consideration.

Through years of experience in sample bank construction, operation and informatization, CU-5 Biological Sample Bank recognized the bottleneck of high-throughput biological sample processing, and therefore, while bidding for SBS cryopreservation tubes, it has made it a mandatory requirement to adapt the automatic whole-plate cap opener. There are 10 cryopreservation tube manufacturers and agents and 7 whole plate opener manufacturers and agents participating in the bidding. During the on-site testing, some companies were not selected because of unsatisfactory results, despite the fact that they offered free use and other conditions in the business. By virtue of its excellent performance and stability under high intensity use, BioBest’s cryopreservation tubes and equipments became the first domestic cryopreservation tubes with domestic whole plate opener to win the bidding in the domestic public bidding process.

At present, the scientific services market is highly concentrated, the head of the market is firmly held by foreign enterprises, relying on strong R & D advantages, complete product lines and globalization of the operating network, these international giants long-term monopoly of the global and Chinese markets, especially the high-end products localization rate is extremely low, the domestic substitution of a wide space. In terms of the whole plate opener, the market price of imported products is mostly in the range of 600,000-1,000,000 RMB, and the final price is not less than 400,000 RMB. BM series of automatic whole plate opener has been recognized by domestic and foreign users in terms of performance, at the same time, from hardware to software completely independent research and development also makes BM have the ability to meet customer needs, the rapid iteration of the product. For example, in the project of the Fifth Hospital of CUHK, for the user’s demand for internal disinfection of the machine, the solution of similar products is to remove the shell and then use UV lamps to irradiate, while Baiyin integrates this function directly into the internal body of the machine, which can be accomplished by a single click.

BANN is also negotiating with the manufacturers of fully automated liquid handling workstations, and in the future, we will realize the seamless connection between cryopreservation tubes and sample pre-processing through the data interoperability and feeding drive unit, so as to reduce the repetitive physical labor of experimental personnel, and devote our energy to scientific research and experimental process design, and play the role of high-level talents.

During the bidding process, BestBio received strong support from local agent Zhuhai Tongtrends Medical Service Co! We are willing to expand the domestic laboratory equipment/consumables market with our friends and agents around the world to create a better tomorrow for the laboratory science service market.

BANN Counseling | The 15th CNAS accredited biospecimen repository in China!

BANN — Sat, 13 May 2023 10:58:46 +0000

In December 2023, the Biological Sample Bank of Zhejiang Cancer Hospital officially obtained the Certificate of Biological Sample Bank Accreditation (BB0015) issued by the China National Accreditation Service for Conformity Assessment (CNAS), becoming the 15th accredited biospecimen bank organization, and the second biospecimen bank in Zhejiang to obtain this accreditation.

In the traditional way, the codes are usually marked on the side walls. Because the containers are obscured from each other, this method can only read the container codes one by one. By placing pre-made codes on the bottom of the containers, the obstruction can be avoided and up to 96 containers can be decoded at the same time using a batch decoding device. In addition, the batch decoder also analyzes the location of the containers and integrates the code and location into a standard data file.

Hangzhou Biobank Biotechnology Co., Ltd. provided complete accreditation consulting services for the accreditation of the biospecimen bank of Zhejiang Cancer Hospital.

The international standard ISO 20387 General Requirements for Biospecimen Repositories was officially released in August 2019.On August 30, 2019, the State Administration for Market Supervision and Regulation (the National Standardization Administration) issued Chinese National Standard Bulletin No. 10 of 2019, approving GB/T 37864-2019 General Requirements for Biospecimen Repositories Quality and Competence (Equivalent to the adoption of ISO 20387:2018) national standard released for implementation.2021 China National Accreditation Service for Conformity Assessment (abbreviation: CNAS), the authoritative organization for national accreditation and evaluation activities, has formulated the Guidelines for the Accreditation of Biological Sample Banks for Quality and Competence CNAS-CL10 based on the above standard, and has begun to carry out the work of accreditation of biosample banks for quality and competence, which is aimed at enhancing the work of all parties on the preservation of biospecimens It contains requirements for the construction of biospecimen repositories. These requirements enable biobanks to confirm that they are competent to operate as biobanks and have the ability to provide biospecimens and related data of the quality required for research and development. more than 10 biobanking organizations have been accredited in more than three years. The construction of these biobanks is at the forefront of human genetic resources conservation organizations in China.

BANN’s accreditation consultant team has been involved from the beginning of the accreditation program, firstly, they have a complete understanding of the actual operation of the hospital’s biospecimens, conducted a one-week accreditation standard publicity and training for the leadership and staff of the biospecimen repository, and worked with the biospecimen repository to formulate the accreditation program.

The consultant team has an in-depth understanding of the current operation of each department of the biobank, on the basis of which it clarifies the characteristics of the workflow and business of each department, combines with the management basis and background of the organization of the biobank, and carries out a special study, planning and design of the management system of the biobank in accordance with the requirements of the standard. The consultant team, together with the biobanking team, designed and established a biobanking quality management system based on the ISO20387 standard, set up the quality policy and objectives of the biobanking organization, and formulated and prepared four levels of quality management system documents, including the management manual, procedure documents and working documents, etc. More than 300 documents and records were prepared. The first version of the complete biobank quality system document was completed in 2021.

Subsequently, the Biobank’s accreditation consultant team assisted the Biobank to start the trial operation of the system, adopting the “one-on-one” coaching mode to follow up and review all aspects of the Biobank’s operation and to check the gaps and make up for the deficiencies. Despite the impact of the epidemic, the consultant team still successfully completed all the work during the one-year trial operation stage, and helped the biobank to continuously improve the quality management system and complete the assessment, so that the biobank entered the formal accreditation application stage at the end of 2022. Finally, the biobank was successfully accredited in 2023.

In September 2023, the Biological Sample Bank of Zhejiang Cancer Hospital ushered in the on-site evaluation for accreditation. During the three-day on-site inspection, the accreditation experts conducted a full-element review and inspection of the quality and capability of the sample bank construction based on CNAS accreditation guidelines. By means of quality document system audit, on-site inspection, personnel competence assessment, comparison experiment, on-site Q&A, the four experts had full communication with the personnel of the sample bank, and carried out a serious and detailed evaluation and put forward valuable opinions and suggestions for the whole process of sample reception, preparation, storage and distribution, as well as the whole elements of man, machine, material, method and environment, etc., and made an in-depth audit on the operation of the quality system of the sample bank and the technical competence. The quality system operation and technical capability of the biosample bank were reviewed in depth. At the last meeting of the on-site evaluation, Prof. Chen Qubo, the head of the evaluation team, declared that the quality management system and safeguard ability of the biospecimen bank of Zhejiang Cancer Hospital meets the requirements of CNAS “Guidelines for Accreditation of Biospecimen Banks for Quality and Competence”, and the on-site evaluation session was passed successfully.

This accreditation has enhanced the awareness of all the staff of the biosample bank of Zhejiang Cancer Hospital on the construction of quality system, and also marked the continuous and effective operation of the quality management system of the biosample bank of Zhejiang Cancer Hospital. At the same time, it promotes the standardization of biological samples preservation, collection and sharing, provides important guidance for the comprehensive optimization of resource sharing services, and is an important manifestation of the establishment and application of quality management system for high-quality clinical biological samples and standardization of technical and service capabilities. Zhejiang Cancer Hospital Biological Sample Bank will continue to improve the level of quality management and technical service, further form a standardized, standardized, informationized, featured boutique and shareable biological sample research and support system, and strive to provide a standardized technical platform and diversified resource support for the hospital’s various clinical and translational medical researches; and at the same time, as the hospital’s three-in-one scientific research service of “Basic, Clinical and Translational”, it will provide a comprehensive and comprehensive service to optimize the resource sharing. At the same time, as one of the core components of the hospital’s “basic, clinical and translational” three-in-one scientific research service platform, it will work together with various research platforms to help the hospital’s innovative and high-quality development of clinical research and translational medicine.

The consulting team of Biobanking is composed of several experts who have been engaged in the foundation and quality management of the sample bank field for the earliest time in China, participated in the national project of biosample bank and formulated a number of standards related to the field of biosample bank, and authored the earliest monograph on quality management of biosample bank in China, “Capacity Building and Best Practices of Biological Specimen Banks” (published by Science Publishing House in 2013).

This is a English demo

BANN — Wed, 14 Jun 2017 14:35:55 +0000

2024年5月27日，杭州市经信局（数字经济局）发布通知，经过专家评审，包括杭州百伴生物技术有限公司在内的13家企业，获批首批企业数改项目备案。

传统方式的编码通常标记在侧壁。因为容器相互遮挡，导致这种方式只能逐一读取容器编码。将预制编码制作在容器底部可以避免遮挡，使用批量解码设备可以同时完成多达96只容器的解码。不仅如此，批量解码器同时还解析容器所在位置，将编码与位置整合形成标准数据文件。

百伴生物自2014年企业成立以来，就一直对主营产品SBS冻存管进行严格的指控标准。2016年完成了ISO 9001质量体系认证, ISO 14001环境管理体系认证；2017年完成了欧盟CE标准认证，ISO13485医疗器械质量管理体系认证。

为了进一步保证产品质量的精度与产品的稳定性、一致性。百伴生物于2018年开始对生产体系进行全面的自动化改造，经过5年的不断改进与迭代，公司已经实现了包括自动生产、自动装配、自动打码、自动装盒、视觉识别检测等一系列生产流程的自动化，在提高生产效率、保证产品质量的同时，有效地减少了因为人工操作而导致的产品不良率。

2023年，百伴生物在生产自动化的前提下，开展了公司的数字化改造。不仅将生产设备通过物联网技术进行全面整合、管理，还进一步打通了生产、销售、财务之间的数据传递，将数字化管理贯穿到整个企业管理的各个环节，实现了物料流转、工时管理、营销管理、成本控制等各个方面的量化/可视化管理。

经过各级经信部门的推荐以及最终的专家评选，百伴生物荣幸地获得了“数字之城”杭州市的首批13家数改项目备案企业之一，百伴生物将以此为契机，继续对企业管理进行更深层次的数字化改造，努力练好内功、打好基础，不因企业规模的扩大而丧失对产品质量的把控、对企业经营各个环节的把控，进一步做大做强，力争成为杭州市数改项目的名片！